LAB -Sterilization and Disinfection

STERILIZATION AND DISINFECTION

HIV is a very fragile virus. Boiling for few seconds kills the virus. Most of the disinfectants used in hospital easily kill HIV . But disinfection and cleaning with water is essential before sterilization/Disinfection to ensure viral inactivation.


Sterilization

• Autoclaving at 121(deg) C, 15  lbs pressure for 20 minutes.
• Dry heat 160(deg)C, for 1 hr.
• Boiling for 20-30 minutes.

  Do not add instruments/water during sterilization cycle.

Chemical disinfection


Sodium hypochlorite,Lysol, Chloramine, ethanol, 2-Propanol , Formalin.Glutaraldehyde,Povidone iodine (PVI), Hydrogen peroxide.